ACELL September 46/3
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چکیده
Hecht, Gail, and Athanasia Koutsouris. Myosin regulation of NKCC1: effects on cAMP-mediated Cl2 secretion in intestinal epithelia. Am. J. Physiol. 277 (Cell Physiol. 46): C441–C447, 1999.—The basally located actin cytoskeleton has been demonstrated previously to regulate Cl2 secretion from intestinal epithelia via its effects on the Na1-K1-2Cl2 cotransporter (NKCC1). In nontransporting epithelia, inhibition of myosin light chain kinase (MLCK) prevents cellshrinkage-induced activation of NKCC1. The aim of this study was to investigate the role of myosin in the regulation of secretagogue-stimulated Cl2 secretion in intestinal epithelia. The human intestinal epithelial cell line T84 was used for these studies. Prevention of myosin light chain phosphorylation with the MLCK inhibitor ML-9 or ML-7 and inhibition of myosin ATPase with butanedione monoxime (BDM) attenuated cAMP but not Ca21-mediated Cl2 secretion. Both ML-9 and BDM diminished cAMP activation of NKCC1. Neither apical Cl2 channel activity, basolateral K1 channel activity, nor Na1-K1-ATPase were affected by these agents. Cytochalasin D prevented such attenuation. cAMP-induced rearrangement of basal actin microfilaments was prevented by both ML-9 and BDM. The phosphorylation of mosin light chain and subsequent contraction of basal actin-myosin bundles are crucial to the cAMP-driven activation of NKCC1 and subsequent apical Cl2 efflux.
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ACELL September 46/3
Dodd, J. S., J. A. Raleigh, and T. S. Gross. Osteocyte hypoxia: a novel mechanotransduction pathway. Am. J. Physiol. 277 (Cell Physiol. 46): C598–C602, 1999.—Bone is a unique tissue in which to examine mechanotransduction due to its essential role in weight bearing. Within bone, the osteocyte is an ideal cellular mechanotransducer candidate. Because osteocytes reside distant from the blood supp...
متن کاملACELL September 46/3
Zeiske, Wolfgang, Ilse Smets, Marcel Ameloot, Paul Steels, and Willy Van Driessche. Intracellular pH shifts in cultured kidney (A6) cells: effects on apical Na1 transport. Am. J. Physiol. 277 (Cell Physiol. 46): C469–C479, 1999.—We report, for the epithelial Na1 channel (ENaC) in A6 cells, the modulation by cell pH (pHc) of the transepithelial Na1 current (INa), the current through the individu...
متن کاملACELL September 46/3
Shepard, Allan R., and James L. Rae. Electrically silent potassium channel subunits from human lens epithelium. Am. J. Physiol. 277 (Cell Physiol. 46): C412–C424, 1999.—We describe the cloning and characterization of the first human members, hKv9.1 and hKv9.3, of the electrically silent delayed-rectifying-like K1 channel subfamily. Their modulatory effects on the electrically active subfamily m...
متن کاملACELL September 46/3
Stevens, Randel J., Jeffery S. Weinert, and Nelson G. Publicover. Visualization of origins and propagation of excitation in canine gastric smooth muscle. Am. J. Physiol. 277 (Cell Physiol. 46): C448–C460, 1999.—The origin and spread of excitation were visualized with fluo 3 fluorescence in tissues isolated from canine gastric antrum. Sheets of circular muscle (5 3 6 mm) had at least 1 (30%) and...
متن کاملACELL August 46/2
JOHN M. PARK,1 ROSALYN M. ADAM,1 CRAIG A. PETERS,1 PAUL D. GUTHRIE,1 ZIJIE SUN,2 MICHAEL KLAGSBRUN,3 AND MICHAEL R. FREEMAN3 1Urologic Laboratory, Department of Urology, 3Laboratory for Surgical Research, Children’s Hospital, and Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115; and 2Departments of Surgery and Genetics, Stanford University School of Medicine, Stanford,...
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